大同大學 教職員資料 (Information of TTU Faculty & Staff)
 
基本資料 (Basic Info)
姓名:官宜靜 (Kuan, I-Ching) 副教授
單位:生物工程學系  
電話:分機3305接28  
手機:6328  
email:iching@ttu.edu.tw  
網址:  
 
學歷 (Education)
美國 賓州州立大學 生物化學和分子生物 博士 (1986/8 ~ 1993/12)
台灣大學 植物病蟲害學系 學士 (1981/9 ~ 1985/6)
 
經歷 (Experience)
中央研究院 分子生物研究所 博士後研究 (1996/1 ~ 1999/7)
 
專長 (Specialty)
基因工程、酵素固定化 genetic engineering、 protein immobilization
 
開授課程 (Course)
生物技術概論 An introduction to biotechnology
應用微生物技術 Applied microbial biotechnology
基因世界的探索 exploration of the gene world
普通生物實驗 General biology experiment
微生物 Microbiology
微生物實驗 Microbiology experiment
遺傳工程技術與應用 Techniques and applications in genetic engineering
 
獎勵 (Award)
96學年度優良教師 ( 2008-10-07)
94學年度優良導師 (大同大學 2006-10-05)
 
服務 (Service)
布袋戲社指導老師 (2008-09-01 ~ 今)
通識教育課程委員會 (2007-09-01 ~ 今)
品茗社指導老師 (2007-02-01 ~ 今)
學生申訴評議委員會 (2005-08-01 ~ 今)
大學部導師 (2002-09-01 ~ 今)
學生申訴評議委員會 (2003-08-01 ~ 2004-07-31)
研究所導師 (2000-09-01 )
 
研究計畫 (Project)
固定於多巴胺包覆之磁性和銀奈米粒子的D型胺基酸氧化酵素的生化特性
科技部 執行期間:2017-08-01 ~ 2018-07-31
以共固定化的Rhodosporidium toruloides D型胺基酸氧化酶和催化酶生產4-甲硫基-2-氧丁酸
大同大學 B106-S01-024 執行期間:2017-01-01 ~ 2017-11-30
以固定化Trigonopsis variablilis D型胺基酸氧化酵素生產4-甲硫基-氧丁酸
大同大學 B105-S01-008 執行期間:2016-01-01 ~ 2016-11-30
提高重組蛋白的產量
永信藥品工業股份有限公司 E10411-S04-032 執行期間:2015-11-15 ~ 2017-11-14
以泛素催化Haematonectria haematococca D型胺基酸酶的仿生矽化
大同大學 B104-S04-013 執行期間:2015-01-01 ~ 2015-11-30
以泛素-R5融合蛋白催化Haematonectria haematococca D型胺基酸酶的生物矽化
大同大學 B103-S01-010 執行期間:2014-01-01 ~ 2014-12-31
以聚多巴胺包覆的奈米磁珠固定D型胺基酸氧化酵素
大同大學 B101-S02-015 執行期間:2012-10-01 ~ 2013-12-31
以silaffin 導引之仿生矽化固定真菌D 型胺基酸氧化酶
國科會 NSC 101-2221-E-036-030- 執行期間:2012-08-01 ~ 2013-07-31
融合幾丁質結合域之Rhodosporidium toruloides D型胺基酸氧化酵素的生化特性探討
大同大學 B100-S01-022 執行期間:2011-09-01 ~ 2012-07-15
Haematonectria haematococca D型胺基酸氧化酵素的基因選殖
大同大學 B99-S05-031 執行期間:2010-09-01 ~ 2011-07-15
重組Haematonectria haematococca D-amino acid oxidase的生化特性和生產研究
國科會 NSC 99-2221-E-036-024 執行期間:2010-08-01 ~ 2011-07-31
以原生透以原生透明顫菌血紅蛋白和其二聚體異源共表現納豆激酶於大腸桿菌中
大同大學 B-98-S04-016 執行期間:2009-09-01 ~ 2010-07-15
斑馬魚D型胺基酸氧化酵素於大腸桿菌的異源表現與固定化研究
國科會 NSC 98-2221-E-036-019- 執行期間:2009-08-01 ~ 2010-07-31
具轉酯活性脂解酵素基因的選殖與異源表現
大同大學 B97-S04-017 執行期間:2008-09-01 ~ 2009-07-15
以重組脂肪分解酵素生產生質柴油之研究
國科會 NSC 97-2221-E-036-015 執行期間:2008-08-01 ~ 2009-07-31
改善納豆激酉每於大腸桿菌異源表現之研究
大同大學 B96-S06-062 執行期間:2007-09-01 ~ 2008-07-15
D型胺基酸氧化酵素熱穩定性和抗過氧化氫能力的改善
國科會 NSC 95-2221-E-036-043 執行期間:2006-09-01 ~ 2007-08-31
對透明顫菌血紅素與Trigonopsis variabilis D型胺基酸氧化酵素融合蛋白生化特性之評估
大同大學 B95-S07-069 執行期間:2006-09-01 ~ 2007-07-15
從黃豆醱酵食品中篩選可分解纖維蛋白的菌株
大同大學 B94-S05-066 執行期間:2005-09-01 ~ 2006-07-15
以定點定位突變改善Rhodosporidium toruloides D型胺基酸氧化酵素抵抗過氧化氫傷害的能力
大同大學 B93-S05-059 執行期間:2004-09-01 ~ 2005-07-15
Candida boidinii D型胺基酸氧化酵素之基因工程與表現
大同大學 B9208-S07-075 執行期間:2003-09-01 ~ 2004-07-15
Rhodosporidium toruloides D型胺基酸氧化酵素於Pichia pastoris中之異源表現研究
大同大學 B91-S06-54 執行期間:2002-09-01 ~ 2003-07-15
D型胺基酸氧化酵素異源表現最適化之研究
大同大學 B90-1900-09 執行期間:2001-09-01 ~ 2002-07-15
重組微生物D-胺基酸氧化酵素的生產研究(二)
國科會 NSC 90-2214-E-036-009- 執行期間:2001-08-01 ~ 2002-07-31
具高D型胺基酸氧化酵素活性之重組大腸桿菌的篩選
大同大學 B89-1900-02 執行期間:2000-09-01 ~ 2001-07-15
重組微生物D-胺基酸氧化酵素的生產研究((一)
國科會 NSC 89-2214-E-036-018- 執行期間:2000-08-01 ~ 2001-07-31
 
專 利 (Patent)
游吉陽 、李綉鈴、官宜靜、莊喬安, "以仿生物矽化反應提升酵素活性之方法及其套組," 中華民國發明專利第 I 419972號 Dec 2013
 
期刊論文 Refereed Paper
1. I-Ching Kuan, Wei-Chen Kao, Chun-Ling Chen, and Chi-Yang Yu, "Microbial Biodiesel Production by Direct Transesterification of Rhodotorula glutinis Biomass," Energies, Vol.11, pp. 1036, Apr. 2018 SCI 
2. I-Ching Kuan, Sing-Yu Chen, Sheng-Yuan Chang, and Chi-Yang Yu, "Immobilization of D-amino acid oxidase via a biomimetic coating and its application for the production of 4-methylthio-2-oxobutyric acid," Journal of the Taiwan Institute of Chemical Engineers, Vol. 79, pp. 60-65, Oct. 2017 SCI 
3. Ming-Hong Xu, I-Ching Kuan, Fu-Yin Deng, Shiow-Ling Lee, Wei-Chen Kao, and Chi-Yang Yu, "Immobilization of lipase from Candida rugosa and its application for the synthesis of biodiesel in a two-step process," Asia-Pacific Journal of Chemical Engineering, Vol. 11, pp. 910-917, Nov. 2016 SCI 
4. Chi-Yang Yu, Liang-Yu Huang, I-Ching Kuan, and Shiow-Ling Lee, "Optimized production of biodiesel form waste cooking oil by lipase immobilized on magnetic nanoparticles," International Journal of moleuclar sicences, Vol.14, pp. 24074-24086, Dec. 2013 SCI 
5. I-Ching Kuan, Chia-Chi Lee, Bing-Hong Tsai, Shiow-Ling Lee, Wei-Ting Lee, and Chi-Yang Yu, "Optimizing the Production of Biodiesel Using Lipase Entrapped," Energies, Vol. 6, pp. 2052-2064, Apr. 2013 SCI 
6. Chia-Feng Lo, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Optimization of lipase production by Burkholderia sp. using response surface methodology," International Journal of Molecular Sciences, Vol. 13, pp. 14889-14897, Nov. 2012 SCI 
7. I-Ching Kuan, Chiao-An Chuang, Shiow-Ling Lee, and Chi-Yang Yu, "Alkyl-substituted methoxysilanes enhance the activity and stability of D-amino acid oxidase encapsulated in biomimetic silica," Biotechnology Letters, Vol. 34, pp. 1493-1498, Jul. 2012 SCI 
8.官宜靜、王信富、陳怡靜, "仿生矽化技術於酵素固定化的應用," 化工技術, Vol. 220, pp.86-99, July 2011
9. Guan-Chun Chen, I-Ching Kuan, Jian-Ren Hong, Bing-Hong Tsai, Shiow-Ling Lee, and Chi-Yang Yu, "Activity enhancement and stabilization of lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica," Biotechnology letters, Vol. 33, pp.525-529, March 2011 SCI 
10. I-Ching Kuan, Jian-Cheng Wu, Shiow-Ling Lee, Chen-Wei Tsai, Chiao-An Chuang, and Chi-Yang Yu, "Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica," Biochemical Engineering Journal, Vol. 49, No. 3, pp. 408-413, May 2010 SCI 
11. Hao-Chieh Hsieh, I-Ching Kuan, Shiow-Ling Lee, Gee-Yeng Tien, Yi-Jen Wang, and Chi-Yang Yu, "Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles," Biotechnology letters, Vol. 31, pp.557-563, Apr. 2009
12. Shih-Juei Wang, Chi-Yang Yu, and I-Ching Kuan, "Stabilization of native and double D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis by immobilization on streptavidin-c," Biotechnology letters, Vol. 30, pp. 1973-1981, Nov. 2008 SCI 
13. Iching Kuan, Weiguo Gu, Jiancheng Wu, Chehung Wei, Koshao Chen, and Chiyang Yu, "Simple surface treatments enhance amplification efficiency in a poly(dimethylsiloxane)-based flow-through PCR device," Chemical Engineering Journal, Vol. 143, pp. 326-330, Sep. 2008 SCI 
14. Shih-Juei Wang, Chi-yang Yu, Cheng-Kang Lee, Ming-Tai Chern, and I-Ching Kuan, "Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to hydrogen peroxide," Biotechnology letters, vol. 30, pp.1415-1422, 2008 SCI 
15. Iching Kuan, Renjie Liao, Haochieh Hsieh, Kuanchun Chen, and Chiyang Yu, "Properties of Rhodotorula gracilis D-amino Acid Oxidase Immobilized on Magnetic Beads through His-tag," J Biosci Bioen, Vol. 105, No. 2, pp. 110-115, Feb. 2008 SCI 
16. Chiyang Yu, Wenshian Liang, Iching Kuan, Chehung Wei, and Weiguo Gu, "Fabrication and Characterization of a Flow-Through PCR Device with Integrated Chromium Resistive Heaters," Journal of the Chinese Institute of Chemical Engineers, Vol. 38, pp. 333-339, 2007 SCI 
17. Chi-Yang Yu, Mao-Lung Liu, I-Ching Kuan, and Shiow-Ling Lee, "Low-Density Protein Microarray on Nitrocellulose Membrane," J Biomed Lab Sci, Vol. 18, No1~4, pp15-22, Sep. 2006
18.Chien, L.-J., J.-M. Wu, I.-C. Kuan and C.-K. Lee, "Coexpression of Vitreoscilla Hemoglobin Reduces the Toxic Effect of Expression of D-Amino Acid Oxidase in Escherichia coli," Biotechnol. Prog., Vol. 20, No. 5, pp. 1359-1365, Oct. 2004 SCI 
19.Chern, M.-K., T. C. Wu, C.-H. Hsieh, C.-C. Chou, L.-F. Liu, I-C. Kuan and Y.-H. Yeh, "Tyr115, Gln165 and Trp209 contribute to the 1,2-epoxy-3-(p-nitrophenoxy)propane-conjugating activity of glutathione S-transferase cGSTM1-1.," J. Mol. Biol, Vol. 300, pp. 1257-1269, 2000 SCI 
20.Hwang, Debbie D.-W., L.-F. Liu, I-C. Kuan, L.-Y. Lin, T.-C. S. Tam and M. F. Tam, "Co-expression of glutathione S-transferase with methionine aminopeptidase: a system of producing enriched N-terminal processed proteins in Escherichia," Biochem. J., Vol. 338, pp. 335-342, 1999 SCI 
21.Sun, Y.-J., I-C. Kuan, M. F. Tam and C.-D. Hsiao, "The three-dimensional structure of an avian class-mu glutathione S-transferase, cGSTM1-1 at 1.94 Å resolution.," J. Mol. Biol., Vol. 278, pp. 239-252, 1998 SCI 
22.Kuan, I-C., K. A. Johnson and M. Tien, "Kinetic analysis of manganese peroxidase: the reaction with manganese complexes.," J. Biol. Chem., Vol. 268, No. 27, pp. 20064-20070, Sep. 1993 SCI 
23.Banci, L., I. Bertini, I-C. Kuan, M. Tien, P. Turano and A. J. Vila, "NMR investigation of isotopically labeled cyanide derivatives of lignin peroxidase and manganese peroxidase.," Biochemistry, Vol. 32, No. 49, pp. 13483-13489, 1993 SCI 
24.Kuan, I-C. and M. Tien, "Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: activity in the absence of added hydrogen peroxide.," Arch. Biochem. Biophys., Vol. 302, No. 2, pp. 447-454. May 1993 SCI 
25.Kuan, I-C. and M. Tien, "Stimulation of Mn peroxidase activity: a possible role for oxalate in lignin biodegration.," Pro. Natl. Acad. Sci. U. S. A., Vol. 90, 1242-1246, Feb. 1993 SCI 
26.Kuan, I-C., D. Cai and M. Tien, " The lignin-degrading system of the wood-destroying fungus Phanerochaete chrysosporium.," Curr. Topics plant Physiol., Vol. 6, pp. 180-192, 1991
27.Kuan, I-C. and M. Tien, "Phosphorylation of lignin peroxidases from Phanerochaete chrysosporium: identification of mannose 6-phosphate.," J. Biol. Chem., Vol. 264, No. 34, pp. 20350-20355, Dec. 1989 SCI 
28. Andrawis, A., E. A. Pease, I-C. Kuan, E. Holzbuaur, and M. Tien, "Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium.," Biochem. Biophys. Res. Commun., Vol.162, pp.673-680, Jul. 1989 SCI 
 
國際研討會論文 International Conference Paper
1. I-Ching Kuan, Chun-Ling Chen, Shiow-Ling Lee, and Chi-Yang Yu, "Microbial biodiesel production by acid-catalyzed direct transesterification of Rhodotorula glutinis biomass," YABEC 2017, pp. 109, Oct. 2017
2. I-Ching Kuan, Sing-Yu Chen, Chi-Yang Yu, and Shiow-Ling Lee, "Enzymatic production of 4-methylthio-2-oxobutyric acid using Trigonopsis variabilis D-amino acid oxidase immobilized on polydopamine-coated magnetic nanoparticles," YABEC 2016, PD-6, pp. 141
3. Chia-Jung Chou, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Protective effect of Mimosa pudica extracts on UVB-induced damage in human dermal fibroblasts," YABEC 2016, PA-16, pp. 53
4. I-Ching Kuan, Li-Ming Huang, Chi-Yang Yu, and Shiow-Ling Lee, "Biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase catalyzed by ubiquitin," Proceedings of YABEC 2015 symposium, p. 88, Oct. 2015
5. Yu-An Huang, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Enhancement of 2-phenylthanol production by Pichia fermentans L-5 with in situ product recovery technique," YABEC 2014, pp.83, Nov. 2014
6. Ming-Hong Xu, Fu-Yin Deng, Shiow-Ling Lee, I-Ching Kuan, and Chi-Yang Yu, "Toward an efficient conversion of waste cooking oil to biodiesel:characteriztion of Candida rugosa lipase immobilized on diaion HP-20," YABEC 2014, pp. 189, Nov. 2014
7. Chi-Yang Yu, Liang-Yu Huang, I-Ching Kuan, and Shiow-Ling Lee, "Optimized production of biodiesel from waste cooking oil by lipase immobilized on magnetic nanoparticles," 2013 Joint of Japan/ Taiwan/ Korean Chemical Engineering Conference & Kyushu Seminar, p. 109-110, Nov. 2013
8. I-Ching Kuan, Cheng-Bin Zheng, Chi-Yang Yu, and Shiow-Ling Lee, "Enzymatic properties of an E. coli-expressing Burkholderia lipase," YABEC 2013, pp. 99, Aug. 2013
9. I-Ching Kuan, Tzu-Ting Liao, Chi-Yang Yu, and Shiow-Ling Lee, "Stabilization of Rhodosporidium toruloides D-amino acid oxidase via biosilicification catalyzed by the chimeric protein of ubiquitin and R5 silaffin," WCCE9&APCChE 2013, pp.074, Aug. 2013
10. I-Ching Kuan, Shin-Fu Wang, Chi-Ching Yu, and Shiow-Ling Lee, "Immobilization of Rhodosporidium toruloides D-amino acid oxidase via silaffin-mediated biomimetic silicification ," The 18th symposium of young asian biochemical engineer's community, pp.59, Oct. 2012
11. Bing-Hong Tsai, Shiow-Ling Lee, I-Ching Kuan, Chia-Chi Lee, and Chi-Yang Yu, "Optimization of biodiesel production using lipase encapsulated in biomimetic silica," The 18th symposium of young asian biochemical engineer's community, pp.62, Oct. 2012
12. Si-Yuan Chen, Ming-Tse Lin, I-Ching Kuan, Chi-Yang Yu, and Shiow-Ling Lee, "Inhibitory effects of Rosa taiwanensis fruits on melanogensis in B16F0 melanoma cells ," The 18th symposium of young asian biochemical engineer's community, pp.125, Oct. 2012
13. I-Ching Kuan, Ko-Lun Chen, Shiow-Ling Lee, and Chi-Yang Yu, "Cloning and co-expression of the lipase and foldase gene from Burkholderia sp. in Escherichia coli," Proceedings of YABEC 2011 Symposium, pp.55, Oct. 2011
14. Shao-Ming Lu, Chi-Yang Yu, I-Ching Kuan, Jia-Fong Luo, and Shiow-Ling Lee, "Purification and characterization of an extracellular lipase from Burkholderia sp.," Proceedings of YABEC 2011 Symposium, pp. 97, Oct. 2011
15. I-Ching Kuan, Jun-Wei Ye, Chi-Yang Yu, and Shiow-Ling Lee, "Biochemical Characterization of Recombinant D-Amino Acid Oxidase from Haematonectria haematococca," Asian Congress on Biotechnology Book of Abstracts, pp. 160, May 2011
16. I-Ching Kuan, Yun-Ru Lee, Chi-Yang Yu, and Shiow-Ling Lee, "Cloning and overexpression in E. coli of a cDNA encoding Haematonectria haematococca D-amino acid oxidase," Proceedings of YABEC 2010 symposium, p.16, Nov. 2010
17. Chiao-An Chuang, I-Ching Kuan, Shiow-Ling Lee, and Chi-Yang Yu, "Bioimprinting of D-amino acid oxidase encapsulated in polyallylamine mediated biomimetic silica," Proceedings of YABEC 2010 symposium, p. 14, Nov. 2010
18. Yi-Chen Liu, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Antioxidant properties of methanol extract from Chamaesyce thymifolia," Proceedings of YABEC 2010 symposium, p.14, Nov. 2010
19. Kuan-Chun Chen, Bing-Hong Tsai, Chen-Wei Tsai, I-Ching Kuan, Shiow-Ling Lee, and Chi-Yang Yu, "Stabilization of lipase from Pseudomonas cepacia by encapsulation in polyallylamine-mediated biomimetic silica," 13th Asia Pacific Confederation of Chemical Engineering Congress, 10401, Oct. 2010
20. I-Ching Kuan, Jhen-Yang Chi, Chi-yang Yu, and Shiow-Ling Lee, "Biochemical characterization of his-tagged zebra fish D-amino acid oxidase expressed in E. coli," Journal of Bioscience and Bioengineering, Vol. 108, No. S1, pp. S112, Nov. 2009 SCI 
21. Kai-Ho Mak, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Some properties of immobilized Pseudomonas cepacia lipase onto magnetic nanoparticles," Journal of Bioscience and Bioengineering, Vol. 108, No. S1, pp.S112-S113, Nov. 2009 SCI 
22. Hao-Chieh Hsieh, I-Ching Kuan, Shiow-Ling Lee, Kai-Ho Mak, Kuan-Chun Chen, Yi-Jen Wang, and Chi-Yang Yu, "Stabilization of D-amino acid oxidase from Rhodotorula gracilis by immobilization onto magnetic nanoparticles," The 14th Symposium of Young Asian Biochemical Engineers' Cmmunity Program and Abstracts, p. 118, Nov. 2008
23. Yi-Chen Liu, I-Ching Kuan, Chi-Yang Yu, and Shiow-Ling Lee, "Evaluation of antioxidant activities of methanol extract of Polygonum chinensis Linn," Book of Abstract of the 12th Asian Pacific Confederation of Chemical Engineering Congress, Vol. 4, pp.164, Aug. 2008
24. I-Ching Kuan, Shih-Juei Wang, Chi-Yang Yu, and Shiow-Ling Lee, "Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by subunit fusion and immobilization on streptavidin-coated magnetic beads," Book of Abstracts of the 12th Asian Pacific Confederation of Chemical Engineering Congress, Vol. 4, pp.31, Aug. 2008
25. I.-C. Kuan, C.-Y. Yu, and H.-C. Kao, "Stabilization of Rhodosporidium toruloides D-amino acid oxidase by immobilization on CNBr-activated sepharose beads and streptavidin-coated magnetic b," Asia Pacific Biochemical Engineering Conference, PII-33, Nov. 2007
26. C.-Y. Yu, R.-J. Liao, I -C. Kuan, and H.-C. Hsieh, "Properties of D-amino acid oxidase immobilized through histidine-tag," Asia Pacific Biochemical Engineering Conference, PII-28, Nov. 2007
27. Ming-Hung Lin, Yi-Fan Shieh, Chin-Hsiung Wang, Hui-Erh Chang, I-Ching Kuan, and Cheng-Chin Chang, "Quantitative Analysis of Procaterol in Human Plasma by ESI LC/MS/MS," Supplement to Journal of The American Society for Mass Spectrometry, Vol. 18, No. 5S, pp.86S, Jun. 2007
28. Yung-Kun Chang and I-Ching Kuan, "Improvement in tolerance to hydrogen peroxide of Rhodosporidium toruloides D-amino acid oxidase," 12th symposium of Young Asian Biochemical Engineers’ Community, p. 126, Nov. 2006
 
區域研討會論文 Domestic Conference Paper
1.游吉陽、官宜靜、李綉鈴、高唯禎、陳君菱、陳詩傑, "紅酵母(Rhodotorula glutinis)以直接轉酯化生成生質柴油," 第七屆 綠色永續生物技術研討會, P-50, Jul. 2016
2. Ming-Hong Xu, I-Ching Kuan, Fu-Yin Deng, Shiow-Ling Lee, and Chi-Yang Yu, "Immobilization of lipase from Candida rugosa and its application for the synthesis of biodiesel in a two-step process," Proceedings of the BEST conference & international symposium on biotechnology and bioengineering, P2-T1-82
3. Shing-Yu Chen, I-Ching Kuan, Shiow-Ling Lee, and Chi-Yang Yu, "Immobilization of D-amino acid oxidase via a biomimetic film and its application for the resolution of D/L-methionine," Proceedings of the BEST conference & international symposium on biotechnology and bioengineering, P2-T1-81
4. Chia-Fang Tsai, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Anti-oxidative activity and proliferation effect of Mimosa pudica on human dermal fibroblasts," Proceedings of the BEST conference & international symposium on biotechnology and bioengineering, P1-T4-26
5.陳星宇、官宜靜、李綉鈴、游吉陽, "Rodosporidium toruloides與Trigonopsis variabilis D型胺基酸氧化酶於磁性奈米粒子之固定化," 2014台灣化學工程學會61週年年會, B_P_N_012, Dec. 2014
6.胡家瑜、周佳蓉、游吉陽、官宜靜、李綉鈴, "含羞草對人類角蛋白細胞經UVB照射後之抗氧化保護作用," 2014台灣化學工程學會61週年年會, B_P_N_015, Dec. 2014
7. I-Ching Kuan, Sheng-Yuan Chang, Chi-Yang, and Shiow-Ling Lee, "Stabilization of D-amino acid oxidase by immobilization onto polydopamine-coated magnetic nanoparticles," 第19屆生物技術暨生物工程研討會, P4-25, pp. 269, Jun. 2014
8. Yu-An Huang, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Optimized production of 2-phenylethanol by pichia fermentans L-5 in a jar fermentor," 第19屆生物技術暨生物工程研討會, P1-48, pp. 186, Jun. 2014
9. Chia-Yu Hu, Chia-Jung Chou, Chi-Yang Yu, I-Ching Kuan, and Shiow-Ling Lee, "Protective effects of Minosa pudica extract against UVB-induced damage in a keratinocyte cell line (HACAT cells) ," 第19屆生物技術暨生物工程研討會, P7-11, pp. 358, Jun. 2014
10. Chia-Jung Chou, Chia-Yu Hu, I-Ching Kuan, Chi-Yang Yu, and Shiow-Ling Lee, "UVB-Induced damage by Minosa pudica extract and collagen generate in human dermal fibroblasts," 第19屆生物技術暨生物工程研討會, P7-14, pp.361, Jun. 2014
11.鄧福尹、徐銘鴻、官宜靜、李綉鈴、游吉陽, "廢食用油藉由酵素水解及化學酯化轉化為生質柴油," 台灣化學工程學會60周年年會暨國科會化學工程學門成果發表會大會手冊, pp.68, Nov. 2013
12.李韋庭、陳星宇、李綉鈴、官宜靜、游吉陽, "廢食用油進行連續是填充床反映生產生質柴油," 台灣化學工程學會60周年年會暨國科會化學工程學門成果發表會大會手冊, pp.68, Nov. 2013
13.蔡佳芳、官宜靜、游吉陽、李綉鈴, "含羞草抑制黑色素細胞B16F0之黑色素生成," 灣, pp. 70, Nov. 2013
14.官宜靜、廖姿婷、陳怡靜, "融合矽氧沉澱胜肽之Trigonopsis variabilis D型胺基酸氧化酶的仿生矽化," 第十七屆生化工程研討會論文集, pp.164, Jun. 2012
15.葉峻瑋、官宜靜、游吉陽、李綉鈴, "於大腸桿菌中異源表現之斑馬魚D型胺基酸氧化酶的生化特性探討," 第十六屆生化工程研討會, pp. 215, June 2011
16.蔡秉宏、李家綺、李綉鈴、官宜靜、游吉陽, "以反應曲面法探討仿生氧化矽包覆酯解酶生產生質柴油最適化," 第十六屆生化工程研討會, p. 239, June 2011
17.呂紹銘、羅家豐、游吉陽、官宜靜、李綉鈴, "Triton X100對Burkholderia sp.脂解酶純化的影響," 第十六屆生化工程研討會, pp.233, June 2011
18.羅家豐、呂紹銘、游吉陽、官宜靜、李綉鈴, "以Burkholderia sp.生產脂解酶之最佳條件," 第十六屆生化工程研討會, pp. 234, June 2011
19.陳思蒝、官宜靜、游吉陽、李糸秀鈴, "傳統中藥材小金櫻果實之抗氧化活性探討," 第十六屆生化工程研討會, pp.264, June 2011
20.施淑觀、官宜靜, "以不同Escherichia coli重組質體異源表現納豆激酉每," 2009年第十四屆生化工程研討會論文摘要集, p. 352, Jun. 2009
21.謝豪傑、官宜靜、李糸秀鈴、麥啟豪、王怡人、游吉陽, "奈米磁性粒子固定D型胺基酸氧化酵素以增進其穩定性," 13th生化工程研討會會議手冊, pp. 229 (PV-8), Jun. 2008
22.麥啟豪、游吉陽、謝豪傑、官宜靜、李糸秀鈴, "固定Pseudomonas cepacia脂解酵素於磁性奈米粒子之最適化條件," 13th生化工程研討會會議手冊, pp. 238 (PV-17), Jun. 2008
23.官宜靜、王詩瑞、游吉陽, "Rhodosporidium toruloides 和Trigonopsis variabilis D 型胺基酸氧化酵素二聚體的生化特性和穩定性," 13th生化工程研討會會議手冊, pp. 231 (PV-10), Jun. 2008
24.官宜靜、高緒均、王詩瑞、游吉陽, "D型胺基酸氧化酵素熱穩定性和抗過氧化氫能力的改善," 台灣化學工程學會第54屆年會暨國科會化工學門成果發表會論文摘要集, pp.80, Nov. 2007
25.謝豪傑、官宜靜、李糸秀鈴、麥啟豪、廖致嘉、陳冠純、游吉陽, "D-型胺基酸氧化酵素固定於奈米磁性粒子之先期研究," 台灣化學工程學會第54屆年會暨國科會化工學門成果發表會論文摘要集, p. 67, Nov. 2007
26.古偉國、官宜靜、游吉陽, "PEO表面修飾對PDMS流道式PCR晶片之影響," 第十一屆奈米工程暨微系統技術研討會, p. 19, Aug. 2007
27.古偉國、游吉陽、官宜靜、魏哲弘, "PDMS微流道PCR晶片之製程與性質探討," 第十二屆生化工程研討會論文摘要集, p. 203, Jun. 2007
28.廖仁傑、游吉陽、官宜靜, "以His-tag方式固定D-型胺基酸氧化酶之性質探討," 第十二屆生化工程研討會論文摘要集, P. 230, Jun. 2007
29.官宜靜、林文山, "以不同啟動子驅動透明顫菌血紅蛋白共同表現對於D型胺基酸氧化於E. coli異源表現的影響," 第十二屆生化工程研討會論文摘要集, p. 232, Jun. 2007
30.林峰毅、官宜靜, "以大腸桿菌異源表現nattokinase," 第十一屆生化工程研討會&生醫奈米暨組織工程國際研討會, p7-073, Jun. 2006
31.簡郁沛、官宜靜、高緒鈞、游吉陽, "以固定化酵素檢測單一核甘酸多型性," 第十一屆生化工程研討會&生醫奈米暨組織工程國際研討會, p. 232, June, 2006
32.游吉陽、魏哲弘、官宜靜、梁文憲、陳瑞鴻、高緒鈞、藍培軒, "整合型PCR 晶片以兩段溫度進行放大," 中華民國九十四年生物醫學工程科技研討會, NSC-P-022
33.李文華、官宜靜, "Rhodosporidium toruloides和Trigonopsis variabilis D型胺基酸氧化酵素在大腸桿菌中表現的最適化," 中國化學工程學會52週年暨國科會化學工程學門成果發表會論文摘要集, pp.87, Nov. 2005
34.梁靖宗、官宜靜, "蜜環菌的抗氧化分析," 台灣食品科學技術學會食品年會第三十五次會員大會手冊, pp. 205, Nov. 2005
35.吳東嶺、吳明鋒、蔡志晃、駱彥甫、官宜靜*, "重組微生物D型胺基酸氧化酵素的生產研究," 中國化學工程學會年會暨專題研討會論文集, p. 8, Nov. 2002
36.吳東嶺、吳明鋒、官宜靜, "重組微生物D-胺基酸氧化酵素的生產研究," 生化工程研討會論文集, Vol. 1, pp.345-350, June 2002
 
研究計劃成果報告 Project Report
1. 官宜靜、施淑觀、葉俊瑋、陳怡靜, 斑馬魚D型胺基酸氧化酵素於大腸桿菌的異源表現與固定化研究, 國科會研究報告, NSC 98-2221-E-036-019, Oct. 2010
2. 官宜靜、施淑觀, 以原生透明顫菌血紅蛋白和其二聚體異源共表現納豆激酶於大腸桿菌中, 基礎案研究報告, B98-S04-016, Aug . 2010
3. 官宜靜、游吉陽、葉峻瑋、陳克綸, 以重組脂肪分解酵素生產生質柴油之研究, 國科會研究報告, NSC NSC 97-2221-E-036 -015, Oct. 2009
4. 官宜靜、陳克綸、葉峻瑋, 具轉酯活性脂解酶基因的選殖與異源表現, 基礎案研究報告, B97-S04-017, Oct. 2009
5. 官宜靜、林峰毅、施淑觀, 改善納豆激酶於大腸桿菌異源表現之研究, 基礎案研究報告, B96-S06-062, Aug. 2008
6. 官宜靜、游吉陽、蔡鎮陽、黃佩萱, D型胺基酸氧化酵素熱穩定性和抗氧化氫能力的改善, 國科會研究報告NSC 95-2221-E-036-043, Oct. 2007
7. 官宜靜, 對透明顫菌血紅素與Trigonopsis variabilis D型胺基酸氧化酶融合蛋白生化特性之評估, 基礎案研究報告 B95-S07-069, Oct. 2007
8. 官宜靜, 從黃豆發酵食品中篩選可分解纖維蛋白的菌株, 基礎案研究報告,B94-S05-066, Oct. 2006
9.官宜靜, 以定點定位突變改善Rhodosporidium toruloides D型胺基酸氧化酵素抵抗過氧化氫傷害的能力, 基礎案研究報告, B93-S05-059, Oct. 2005
10.官宜靜, Candida boidinii D型胺基酸氧化酵素之基因工程與表現, 基礎案研究報告, B9208-S07-075, Oct. 2004
11.官宜靜, Rhodosporidium toruloides D型胺基酸氧化酵素於Pichia pastoris中之異源表現研究, 基礎案研究報告, B 91-S06-054, Oct. 2003
12.官宜靜, D-胺基酸氧化酵素異源表現最適化之研究, 基礎案研究報告, B 90-1900-09, Oct. 2002
13.官宜靜、吳東嶺、吳明鋒、蔡志晃、駱彥甫, 重組微生物D-胺基酸氧化酵素的生產研究, 國科會研究報告, NSC 90-2214-E-036-009, Oct. 2002
14.官宜靜、吳東嶺、吳明鋒, 重組微生物D-胺基酸氧化酵素的生產研究, 國科會研究報告, NSC 89-2214-E-036-018, Oct. 2001
15.官宜靜, 具高胺基酸氧化酵素活性之重組大腸桿菌的篩選, 基礎案研究報告, B 89-1900-02, Oct. 2001