大同大學教師資料

大同大學教職員資料 (Information of TTU Faculty & Staff)

基本資料 (Basic Info)

姓名：官宜靜 (Kuan, I-Ching) 副教授

單位：生物工程學系

電話：分機3305接28

手機：6328

email：iching@ttu.edu.tw

網址：

學歷 (Education)

美國賓州州立大學生物化學和分子生物博士 (1986/8 ~ 1993/12)

台灣大學植物病蟲害學系學士 (1981/9 ~ 1985/6)

經歷 (Experience)

中央研究院分子生物研究所博士後研究 (1996/1 ~ 1999/7)

專長 (Specialty)

基因工程、酵素固定化 genetic engineering、 protein immobilization

開授課程 (Course)

生物技術概論 An introduction to biotechnology

應用微生物技術 Applied microbial biotechnology

基因世界的探索 exploration of the gene world

普通生物實驗 General biology experiment

微生物 Microbiology

微生物實驗 Microbiology experiment

遺傳工程技術與應用 Techniques and applications in genetic engineering

獎勵 (Award)

96學年度優良教師 ( 2008-10-07)

94學年度優良導師 (大同大學 2006-10-05)

服務 (Service)

1.	布袋戲社指導老師 (2008-09-01 ~ 今)
2.	通識教育課程委員會 (2007-09-01 ~ 今)
3.	品茗社指導老師 (2007-02-01 ~ 今)
4.	學生申訴評議委員會 (2005-08-01 ~ 今)
5.	大學部導師 (2002-09-01 ~ 今)
6.	學生申訴評議委員會 (2003-08-01 ~ 2004-07-31)
7.	研究所導師 (2000-09-01 )

專利 (Patent)

1.	游吉陽、李綉鈴、官宜靜、莊喬安, "以仿生物矽化反應提升酵素活性之方法及其套組," 中華民國發明專利第 I 419972號 Dec 2013

學術研究計畫(研4)

1.	固定於多巴胺包覆之磁性和銀奈米粒子的D型胺基酸氧化酵素的生化特性，2017/8/1 ~ 2019/4/30
2.	重組Haematonectria haematococca D-amino acid oxidase的生化特性和生產研究，國科會一般型研究計劃 2010/8/1 ~ 2011/7/31
3.	斑馬魚D型胺基酸氧化酵素於大腸桿菌的異源表現與固定化研究，國科會一般型研究計劃 2009/8/1 ~ 2010/7/31
4.	以重組脂肪分解酵素生產生質柴油之研究，國科會一般型研究計劃 2008/8/1 ~ 2009/7/31
5.	D型胺基酸氧化酵素熱穩定性和抗過氧化氫能力的改善，國科會一般型研究計畫 2006/9/1 ~ 2007/8/31
6.	重組微生物D-胺基酸氧化酵素的生產研究（二），國科會一般型研究計畫 2001/8/1 ~ 2002/7/31
7.	重組微生物D-胺基酸氧化酵素的生產研究(（一），國科會一般型研究計畫 2000/8/1 ~ 2001/7/31

產學合作計畫(研9)

1.	探討不同乳化配方對益生菌的活存率的影響，2022/1/1 ~ 2022/6/30
2.	探討不同乳化配方對益生菌的活存率影響，2021/7/1 ~ 2021/12/31
3.	探討不同乳化配方對益生菌的活存率的影響，2021/1/1 ~ 2021/6/30
4.	aFGF及bFGF大腸桿菌分泌型系統開發，2019/5/1 ~ 2020/4/30
5.	以共固定化的Rhodosporidium toruloides D型胺基酸氧化酶和催化酶生產4-甲硫基-2-氧丁酸，2017/1/1 ~ 2017/11/30
6.	以固定化Trigonopsis variablilis D型胺基酸氧化酵素生產4-甲硫基-氧丁酸，2016/1/1 ~ 2016/11/30
7.	提高重組蛋白的產量，2015/11/15 ~ 2017/11/14
8.	提高大腸桿菌表現之重組rhG-CSF的產量，2015/11/15 ~ 2016/11/14
9.	以泛素催化Haematonectria haematococca D型胺基酸酶的仿生矽化，2015/1/1 ~ 2015/11/30
10.	以泛素-R5融合蛋白催化Haematonectria haematococca D型胺基酸酶的生物矽化，2014/1/1 ~ 2014/12/31
11.	以聚多巴胺包覆的奈米磁珠固定D型胺基酸氧化酵素，2012/10/1 ~ 2013/12/31
12.	融合幾丁質結合域之Rhodosporidium toruloides D型胺基酸氧化酵素的生化特性探討，2011/9/1 ~ 2012/7/15
13.	Haematonectria haematococca D型胺基酸氧化酵素的基因選殖，2010/9/1 ~ 2011/7/15
14.	以原生透以原生透明顫菌血紅蛋白和其二聚體異源共表現納豆激酶於大腸桿菌中，2009/9/1 ~ 2010/7/15
15.	具轉酯活性脂解酵素基因的選殖與異源表現，2008/9/1 ~ 2009/7/15
16.	對透明顫菌血紅素與Trigonopsis variabilis D型胺基酸氧化酵素融合蛋白生化特性之評估，2006/9/1 ~ 2007/7/15
17.	從黃豆醱酵食品中篩選可分解纖維蛋白的菌株，2005/9/1 ~ 2006/7/15
18.	以定點定位突變改善Rhodosporidium toruloides D型胺基酸氧化酵素抵抗過氧化氫傷害的能力，2004/9/1 ~ 2005/7/15
19.	Candida boidinii D型胺基酸氧化酵素之基因工程與表現，2003/9/1 ~ 2004/7/15
20.	D型胺基酸氧化酵素異源表現最適化之研究，2001/9/1 ~ 2002/7/15
21.	具高D型胺基酸氧化酵素活性之重組大腸桿菌的篩選，2000/9/1 ~ 2001/7/15

期刊論文 (Journal Paper)

1.	Shih-Jie Chen, I-Ching Kuan, Yu-Feng Tu, Shiow-Ling Lee, Chi-Yang Yu(2020). Surfactant-assisted in situ transesterification of wet Rhodotorula glutinis. Journal of bioscience and bioengineering, 130(4), 397-401. SCIE(SCI)
2.	I-Ching Kuan, Wei-Chen Kao, Chun-Ling Chen, Chi-Yang Yu(2018). Microbial Biodiesel Production by Direct Transesterification of Rhodotorula glutinis Biomass. Energies, 11(5). SCIE(SCI)
3.	I-Ching Kuan, Sing-Yu Chen, Sheng-Yuan Chang, Chi-Yang Yu(2017). Immobilization of D-amino acid oxidase via a biomimetic coating and its application for the producti. Journal of the Taiwan Institute of Chemical Engineers, 79. SCIE(SCI)
4.	I-Ching Kuan, Sing-Yu Chen, Sheng-Yuan Chang, Chi-Yang Yu(2017). Immobilization of D-amino acid oxidase via a biomimetic coating and its application for the production of 4-methylthio-2-oxobutyric acid. Journal of the Taiwan Institute of Chemical Engineers, 79, 60-65. SCIE(SCI)
5.	Shiow-Ling Lee, Ming-Hong Xu, I-Ching Kuan, Chi-Yang Yu, Wei-Chen Kao, Fu-Yin Deng(2016). Immobilization of lipase from Candida rugosa and its application for the synthesis of biodiesel in a two-step process.. Asia-Pac. J. Chem. Eng.,, 11, 910-917. SCIE(SCI)
6.	I-Ching Kuan, Chia-Chi Lee, Bing-Hong Tsai, Shiow-Ling Lee, Wei-Ting Lee, Chi-Yang Yu(2013). Optimizing the Production of Biodiesel Using Lipase Entrapped. Energies, 6, 2052-2064. SCIE(SCI)
7.	Shiow-Ling Lee, Liang-Yu Huang, I-Ching Kuan, Chi-Yang Yu(2013). Optimized production of biodiesel from waste cooking oil by lipase immobilized on magnetic nanoparticles.. Int. J. Mol. Sci., 14, 24074-2408. SCIE(SCI)
8.	Shiow-Ling Lee, Chia-Chi Lee,, I-Ching Kuan, Chi-Yang Yu, Bing-Hong Tsai, Wei-Ting Lee(2013). Optimizing the production of biodiesel using lipase entrapped in biomimetic silica.. Energies., 6, 2052-2064. SCIE(SCI)
9.	S.-L. Lee, Lo, C.-F., I-Ching Kuan, Chi-Yang Yu(2012). Optimization of lipase production by Burkholderia sp. using response surface methodology.. Int. J. Mol. Sci., 13, 14889-1489. SCIE(SCI)
10.	Shiow-Ling Lee, Chiao-An Chuang, I-Ching Kuan, Chi-Yang Yu(2012). Alkyl-substituted methoxysilanes enhance the activity and stability of d-amino acid oxidase encapsulated in biomimetic silica. Biotechnol Lett, 34, 1493–1498. SCIE(SCI)
11.	S.-L. Lee, K.-H. Tsui, I-Ching Kuan, T.-H. Feng, P.-L. Chang, Chung, L.-C., H.-H. Juang(2012). L-mimosine blocks cell proliferation via upregulation of B-cell translocation gene 2 and N-myc downstream regulated gene 1 in prostate carcinoma cells.. Am. J. Physiol. Cell. Physiol., 302, C676–C685.. SCIE(SCI)
12.	Shiow-Ling Lee, Chen, G.-C., I-Ching Kuan, Chi-Yang Yu, J.-R. Hong, B.-H. Tsai(2011). 2011. Activity enhancement and stabilization of lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica.. Biotechnol. Lett., 33, 525–529. SCIE(SCI)
13.	官宜靜、王信富、陳怡靜（2011）。仿生矽化技術於酵素固定化的應用。化工技術，220，86-99。SCIE(SCI)
14.	Shiow-Ling Lee, Jian-Cheng Wu, I-Ching Kuan, Chi-Yang Yu, Chen-Wei Tsai, Chiao-An Chuang(2010). Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica. Biochem. Eng. J., 49, 408-413. SCIE(SCI)
15.	Shiow-Ling Lee, Hao-Chieh Hsieh, I-Ching Kuan, Chi-Yang Yu, Gee-Yeng Tien, Yi-Jen Wang(2009). Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles. Biotechnol. Lett., 31(4), 557-263. SCIE(SCI)
16.	I-Ching Kuan, Jhen-Yang Chi, Chiyang Yu, Shiow-Ling Lee(2009). Biochemical characterization of his-tagged zebra fish D-amino acid oxidase expressed in E. coli. Journal of Bioscience and Bioengineering, 2009. SCIE(SCI)
17.	Kai-Ho Mak, Chi-Yang Yu, I-Ching Kuan, Shiow-Ling Lee(2009). Some properties of immobilized Pseudomonas cepacia lipase onto magnetic nanoparticles. Journal of Bioscience and Bioengineering, 2009. SCIE(SCI)
18.	Shih-Juei Wang, Chi-Yang Yu, I-Ching Kuan(2008). Stabilization of native and double D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis. Biotechnology Letters, 2008, 1973–1981. SCIE(SCI)
19.	Iching Kuan, Weiguo Gu, JianchengWu, Chehung Wei, Koshao Chen, Chiyang Yu(2008). Effects of grafting poly(ethylene oxide) on the amplification efficiency of a poly(dimethylsiloxane)-based flow-through PCR device. Chemical Engineering Journal, 2008, 326-330. SCIE(SCI)
20.	Shih-Juei Wang, Chi-yang Yu, Cheng-Kang Lee, Ming-Kai Chern, I-Ching Kuan(2008). Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to hydrogen peroxide. Biotechnol. lett., 2008, 1415-1422.
21.	Yu C, Kuan I, Hsieh H, Chen K, Liao R(2008). Properties of Rhodotorula gracilis D-Amino Acid Oxidase. J Biosci Bioeng, 2008, 110-115. SCIE(SCI)
22.	Shih-Juei Wang, Chi-Yang Yu, I-Ching Kuan(2008). Stabilization of native and double D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis by immobilization on streptavidin-c. Biotechnology letters, 30, 1973-1981. SCIE(SCI)
23.	Iching Kuan, Weiguo Gu, Jiancheng Wu, Chehung Wei, Koshao Chen, Chiyang Yu(2008). Simple surface treatments enhance amplification efficiency in a poly(dimethylsiloxane)-based flow-through PCR device. Chemical Engineering Journal, 143, 326-330. SCIE(SCI)
24.	Iching Kuan, Renjie Liao, Haochieh Hsieh, Kuanchun Chen, Chiyang Yu(2008). Properties of Rhodotorula gracilis D-amino Acid Oxidase Immobilized on Magnetic Beads through His-tag. J Biosci Bioen, 105(2), 110-115. SCIE(SCI)

研討會論文 (Conference Paper)

1.	I-Ching Kuan, Chun-Ling Chen, Shiow-Ling Lee, Chi-Yang Yu(2017). Microbial biodiesel production by acid-catalyzed direct transesterification of Rhodotorula glutinis biomass. YABEC 2017（頁 109）. United States of America.
2.	I-Ching Kuan, Sing-Yu Chen, Shiow-Ling Lee, Chi-Yang Yu(2016). Enzymatic production of 4-methylthio-2-oxobutyric acid using Trigonopsis variabilis D-amino acid oxidase immobilized on polydopamine-coated magnetic nanoparticles. YABEC 2016（頁 141）. United States of America.
3.	I-Ching Kuan, Chia-Jung Chou, Shiow-Ling Lee, Chi-Yang Yu(2016). Protective effect of Mimosa pudica extracts on UVB-induced damage in human dermal fibroblasts. YABEC 2016（頁 53）. United States of America.
4.	Chia-Jung Chou, Chi-Yang Yu, I-Ching Kuan, Shiow-Ling Lee(2016). Protective effect of Mimosa pudica extracts on UVB-induced damage in human dermal fibroblasts,. YABEC 2016. United States of America.
5.	I-Ching Kuan, Li-Ming Huang, Chi-Yang Yu, Shiow-Ling Lee(2015). Biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase catalyzed by ubiquitin. Proceedings of YABEC 2015 symposium. United States of America.
6.	I-Ching Kuan, Fu-Yin Deng, Shiow-Ling Lee, Chi-Yang Yu, Ming-Hong Xu(2015). Immobilization of lipase from Candida rugosa and its application for the synthesis of biodiesel in a two-step process. Proceedings of the BEST conference & international symposium on biotechnology and bioengineering. United States of America.
7.	I-Ching Kuan, Shing-Yu Chen, Shiow-Ling Lee, Chi-Yang Yu(2015). Immobilization of D-amino acid oxidase via a biomimetic film and its application for the resolution of D/L-methionine. Proceedings of the BEST conference & international symposium on biotechnology and bioengineering. United States of America.
8.	I-Ching Kuan, Chia-Fang Tsai, Shiow-Ling Lee, Chi-Yang Yu(2015). Anti-oxidative activity and proliferation effect of Mimosa pudica on human dermal fibroblasts. Proceedings of the BEST conference & international symposium on biotechnology and bioengineering. United States of America.
9.	I-Ching Kuan, Yu-An Huang, Shiow-Ling Lee, Chi-Yang Yu(2014). Enhancement of 2-phenylthanol production by Pichia fermentans L-5 with in situ product recovery technique. YABEC 2014（頁 83）. United States of America.
10.	I-Ching Kuan, Ming-Hong Xu, Shiow-Ling Lee, Chi-Yang Yu, Fu-Yin Deng(2014). Toward an efficient conversion of waste cooking oil to biodiesel:characteriztion of Candida rugosa lipase immobilized on diaion HP-20. YABEC 2014（頁 189）. United States of America.
11.	I-Ching Kuan, Tzu-Ting Liao, Shiow-Ling Lee, Chi-Yang Yu(2013). Stabilization of Rhodosporidium toruloides D-amino acid oxidase via biosilicification catalyzed by the chimeric protein of ubiquitin and R5 silaffin. WCCE9&APCChE 2013（頁 74）. United States of America.
12.	I-Ching Kuan, Liang-Yu Huang, Shiow-Ling Lee, Chi-Yang Yu(2013). Optimized production of biodiesel from waste cooking oil by lipase immobilized on magnetic nanoparticles. 2013 Joint of Japan/ Taiwan/ Korean Chemical Engineering Conference & Kyushu Seminar（頁 109-111）. United States of America.
13.	I-Ching Kuan, Cheng-Bin Zheng, Shiow-Ling Lee, Chi-Yang Yu(2013). Enzymatic properties of an E. coli-expressing Burkholderia lipase. YABEC 2013（頁 99）. United States of America.
14.	I-Ching Kuan, Shin-Fu Wang, Shiow-Ling Lee, Chi-Yang Yu(2013). Immobilization of Rhodosporidium toruloides D-amino acid oxidase via silaffin-mediated biomimetic silicification. The 18th symposium of young asian biochemical engineers community（頁 59）. United States of America.